INVESTIGADORES
MAIZTEGUI Barbara
congresos y reuniones científicas
Título:
PI3K mediates islet neogenesis associated protein effect upon B-cell function
Autor/es:
MAIZTEGUI B; ROMAN CL; BORELLI MI; GAGLIARDINO JJ
Lugar:
Chicago
Reunión:
Congreso; 73rd Scientific Sessions American Diabetes Association (ADA); 2013
Institución organizadora:
American Diabetes Association (ADA)
Resumen:
Islet neogenesis associated protein (INGAP-PP) increases insulin secretion (IS) and β-cell mass in normal rats but it is not clear the mechanism by which produces its effects. Thus, we studied INGAP-PP effect on IS, glucose (G) metabolism, glucokinase (GK) activity and protein expression and the PI3K pathway in cultured normal rat islets. Islets were isolated by collagenase digestion and cultured for 4 days in RPMI with 2 g/L NaHCO3, 5% FBS, 1% penicillin/streptomycin and 10 mM G, with or w/o 10 µg/ml INGAP-PP. Islets were preincubated in 3 mM G for 45 min and then incubated with 3, 8 and 16 mM G to measure IS, G metabolism (14CO2 and 3H2O production), GK activity (G-6-P production), GK, IR and PI3K protein expression (Western Blot [WB]) and PI3K/IRS-1 complex (immuneprecipitation and WB). We also studied the effect of Wortmannin (W), a PI3K inhibitor (150 and 300 nM) on G-induced IS in both groups. Results: (C vs. INGAP-PP; *p