Unravelling the photoprotection capacity of resveratrol on his-tidine oxidation

NEYRA RECKY, JAEL R.; DANTOLA, MARIA LAURA; LORENTE, CAROLINA

PHOTOCHEM

MDPI

Año: 2021 vol. 1 p. 209 - 219

2673-7256

The exposure to sun radiation causes great oxidative stress and activate a numerous of defense mechanisms in living systems, such as the synthesis of antioxidants. Resveratrol (RSV), a naturally occurring stilbene molecule, has antioxidant properties and is synthesized in great amount when plants are under high oxidative stress. Likewise, under UV and visible radiation biomolecules are oxidized losing their physiological properties, therefore avoiding the harmful effects of solar radiation is crucial to preserve the functionality of cellular components. In proteins, one essential component that is often susceptible to degradation is the amino acid histidine (His), which can be modified by several oxidizing mechanisms. In this article, we evaluate RSV photoprotection capacity in photosensitized oxidation of His, which is initiated with a one-electron transfer reaction, yielding His radical cation (His?+). The photoprotective properties of RSV were evaluated from kinetics analysis during steady-state irradiation and laser flash photolysis experiments. The experimental results revealed that the presence of RSV in the solution causes an evident decrease of His consumption initial rates as a result from a reaction between His?+ and RSV that recover the amino acid. In addition, we conclude that during its antioxidant action RSV is consumed being a sacrificial antioxidant.