INVESTIGADORES
ROMERO Cintia Mariana
congresos y reuniones científicas
Título:
Ethyl acetate production by immobilized esterases from spore-forming microorganisms
Autor/es:
LOTO F, ROMERO C.M., BAIGORÍ M.D. AND PERA L.M.( 2006).
Lugar:
Rosario. Santa Fe
Reunión:
Congreso; SAIB. 42 Annual Meeting (Argentina).; 2006
Institución organizadora:
saib
Resumen:
ETHYL ACETATE PRODUCTION BY IMMOBILIZED
ESTERASES FROM SPORE-FORMING
MICROORGANISMS
Loto F; Romero CM; Baigorí M; Pera LM
PROIMI-CONICET Av. Belgrano y Pje. Caseros, 4000 Tucumán.
Tel: 0381-4344888
E-mail: lymb@arnet.com.ar
Introduction: Ethyl acetate (EAc) is an important raw material for
many applications in chemical industry including coatings,
adhesives, perfumes, and plasticizers. Enzymatic
biotransformation has the advantage of high specificity and much
simpler processes in comparison to chemical synthesis and
various resolving agents. The aim of this work was to evaluate
the production of EAc using immobilized esterases from sporeforming
microorganisms. Methods: Standard Bacillus subtilis
(168) and spore-forming microorganisms isolated from different
sources (A14, A55, A60, A62, M2) were used throughout this
study. Microorganisms were grown in LB at 37 °C. Both cells
harvested by centrifugation and polyacrylamide gelentrapped
supernatant were used as source of enzyme. Esterifications were
performed during 24h in n-hexane using acetic acid and ethanol
as substrates. Ester synthesis was determined by acid titration
with NaOH. Qualitative analyses of EAc were done by thin layer
chromatography using chloroform as developing solvent. Result
and Conclusion: Good productions of EAc in all systems assayed
were observed. The highest yield was obtained with the
polyacrylamide gelentrapped supernatant from the isolation M2
showing a conversion of 93 %. This result indicates that gel
entrapment method offers a good way for ester synthesis.
This work was supported by grants PIP 6062 and PIP 6203.