INVESTIGADORES
WEINER Andrea Maria Julia
congresos y reuniones científicas
Título:
Deciphering CNBP cellular and molecular function during cranial NC development with a novel morpholino technique
Autor/es:
WEINER ANDREA; ROBERT KELSH; CALCATERRA NORA
Lugar:
Santa Cruz
Reunión:
Congreso; 5th International Meeting of the Latin American Society of Developmental Biology; 2010
Institución organizadora:
LASDB
Resumen:
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Several
issues need to be well thought-out when functionally studying maternally
inherited genes during development. Without considering the use of mutant or
transgenic animals, some loss-of-function methods properly knockdown an mRNA
expression while others block protein function by the expression of dominant
negative isoforms. However, it is known that those methods do not affect the
maternally inherited protein pool and also the dominant negative isoforms are
degraded or titled as development advances, being unable to reduce newly
synthesized proteins. In this work, we report that the use of a morpholino that
specifically impairs splicing of cellular
nucleic acid binding protein (CNBP) pre-mRNA intron 2 leads to the
synthesis of shorter protein isoforms. Furthermore, these appear to act as
dominant negatives, enabling us to elucidate CNBP function during NC
development. Our studies demonstrate that CNBP loss-of-function adversely
affects formation and survival of a subpopulation of the cranial NC cells,
leading to reduction/loss of selected pharyngeal and craniofacial cartilaginous
structures in the developing zebrafish. These defects result from disruption of
CNBP roles in both NC cell survival and specification. The strategy used in
this work may be an interesting experimental tool for studying the role of
other maternally inherited proteins during embryonic development
