ANTIOXIDANT ENZYMES EVALUATION AND PROINFLAMATORY CYTOKINS BEHAVIOR IN ACUTE LEUKEMIA

LAZARTE S.; LEDESMA ACHEM E.; AGÜERO AGUILERA A.; MÓNACO ME.; HARO C.; ISSÉ B.; TERÁN M.

Tafí Viejo

Jornada; XXXVI Jornadas Científicas de la Asociación de Biología de Tucumán; 2019

Asociación de Biología de Tucumán

Different neoplasms show increased levels of reactive oxygen species leading to a proinflammatory and oxidative stress (EOx) state. The aim of the present work was to study biomarkers involved in the antioxidant defenses and inflammatory in acute leukemias (LA). Methods: 47 patients with LA and 36 control subjects (C) were studied at the Institute of Applied Biochemistry of the UNT (August 2016 - December 2018). Gene expression of transcription factor nuclear erythroid factor 2-related factor 2 (Nrf2), antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD), and cytokines TNF-α and IL-6 was evaluated by real-time PCR. Malondialdehyde (MDA) levels, as an indicator of oxidative damage, and CAT activity were determined in serum. Results: Of the total LA studied, 43% were of lymphoid origin, 38% myeloid and 19% promyelocytic (LPA). MDA levels were similar in the groups analyzed. However, higher CAT activity was detected in the LPA (p