Electrochemical Devices for Biomolecular Detection

GONZÁLEZ GRACIELA; PEINETTI ANA SOL; CARUGNO SOFÍA; GÜNTHER MAURICIO; PRIANO GRACIELA; PALLAROLA DIEGO; FERNANDO BATTAGLINI

Playa del Carmen

Conferencia; Zing Electrochemistry Conference; 2009

We report two examples of electrochemical systems aimed to sensor development. One is based on amperometric detection, using an inexpensive eight gold electrode array. The array is manufactured by a combination of screen printing and gold electrodeposition techniques, used with a multipotenciostat, works as an efficient array to develop recognition assays with electrochemical detection. The detection of lipopolysaccharides and peroxidase, as examples of competitive or sandwich assays, will be presented. The other application compromises a nanoporous membrane placed close to the working electrode. In this case the signal is generated by a probe; therefore there is no need for analytes or labeled analytes able to produce an amperometric response. Two types of membranes were studied. The experimental results of the electrochemical system were modeled by numerical simulation. The effect of porous domains in the amperometric response of cyclic voltammetry experiments is simulated to establish the sensitivity of this system to detect molecules able to block the pores. The generated data is in good agreement with experimental results. The experimental sensitivity of the three systems can be observed through the current response of modified surface with anti-HRP-HRP complex.