INVESTIGADORES
TREJO Fernando Miguel
artículos
Título:
Co-culture with potentially probiotic microorganisms antagonizes virulence factors of Clostridium difficile in vitro.
Autor/es:
TREJO F. M., ; PÉREZ P. F. ; DE ANTONI G. L
Revista:
ANTON LEEUW INT. J. G.
Editorial:
SPRINGER
Referencias:
Año: 2010 vol. 98 p. 19 - 29
ISSN:
0003-6072
Resumen:
Toxigenic strains of Clostridium
difficile were co-cultured with different strains of bifidobacteria and lactobacilli.
After centrifugation, spent culture supernatants were tested for biological
activity on cultured Vero cells. Co-culture of Clostridium difficile with some potentially
probiotic strains leads to diminution of the biological activity of spent
culture supernatants. Observed effect cannot be ascribed neither to secreted
factors from probiotics nor to toxin adsorption by bacterial cells. Immunological
assays showed that there was significant diminution of both clostridial toxins
(TcdA and TcdB) in spent culture supernatants of co-cultures as compared with
pure clostridial cultures. Even though co-cultured clostridial cells showed a
slight increase of intracellular toxins, this increase did not completely
explains the diminution of toxin concentration in culture supernatants.
Evidence suggests that the
antagonism could be due to the diminution of the synthesis/secretion of both clostridial
toxins and it could be also related to a decrease of
toxin release from the bacteria.
Our findings provide new
insight on the mechanisms involved in the protective effect of probiotics against
Clostridium difficile.