KETAMINE INDUCES CHANGES IN THE EXPRESSION OF COAT PROTEINS (AP-2) IN NUCLEUS ACCUMBENS OF ADULT RATS

RUIZ AM; CARVELLI L; CAPELLA P; SARTOR T; GIL LORENZO AF; DOMINGUEZ S; SOSA MA

San Juan

Congreso; XXIX Reunión Anual de la Sociedad de Biología de Cuyo; 2011

Clathrin-mediated endocytosis is important in maintainance of membrane homeostasis at nerve terminals. The study of synaptic and clathrin-coat proteins in the central nervous system (CNS) has attracted attention in recent decades, as some illnesses or conditions of nerve injury, accompanied of changes in levels of dopamine and glutamate, affect the expression and localization of these proteins. The activity of drugs such as ketamine in the CNS could involve blocking or changing levels of surface receptors to NMDA. In turn, this could be associated with a change in the internalization of receptors, by alterations in the endocytic machinery at nerve terminals. El objetivo era estudiar los posibles cambios en la expresión y localización de la proteína de la cubierta AP-2 como un índice de cambios en la actividad endocítica, en diferentes áreas del cerebro de rata tras la administración aguda de la ketamina. EscucharLeer fonéticamente. Adult (90 days) Wistar-Kyoto (male) rats were treated intraperitoneally with effective subanesthetic dose of ketamine (10 mg / kg) and sacrificed at 15 min, or 24 hours after. Controls were treated with saline. After dissection, nucleus accumbens (right and left; Accd and Acci) and prefrontal cortex (right and left; Cxd and Cxi) homogenates were obtained and analyzed by Western blot for expression of the adaptor AP-2. Results. The expression of AP2 in Accd treated with ketamine significantly increased over control at 15 min and 24 h (100 ± 13.96 (n = 3) vs. 178.12 ± 15.84 * (n = 4) and 100 ± 15.30 (n = 3 ) vs. 191.16 ± 11.30 * (n = 3) respectively). In turn, the Acci of treated rats shows a significant increase (100 ± 14.00 (n = 3) vs. 179.15 ± 21.54 * (n = 3)) only after 24 hours. In contrast, in the cxd and Cxi were not observed significant changes in AP-2, between control and treated animals. The selective increase in the expression of AP-2 in nucleus accumbens treated with ketamine suggests that the blocking effect of the drug on NMDA receptors alters the endocytic pathway via AP-2, an effect not observed at the level of the prefrontal cortex.