PERSONAL DE APOYO
SARTOR Tirso
artículos
Título:
The effect of the diterpene 5-epi-icetexone on the cell cycle of Trypanosoma cruzi
Autor/es:
ESTEBAN LOZANO; BARRERA PATRICIA; TONN CARLOS; NIETO MATIAS; TIRSO SARTOR; SOSA MIGUEL A
Revista:
PARASITOLOGY INTERNATIONAL
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 2012 vol. 61 p. 275 - 279
ISSN:
1383-5769
Resumen:
Numerous natural compounds have been used against Trypanosoma cruzi, the causative agent of Chagas´ disease.
Here, we studied the effect of the diterpene 5-epi-icetexone on growth and morphology of parasites
synchronized with hydroxyurea, at different periods of time after removal of the nucleotide. We observed
that the diterpene does not affect the growth of the parasites when added within 10 h after removal of hydroxyurea,
but the compound was effective on growth when added to the cultures after 12 h. Thymidine incorporation
was somewhat inhibited when the diterpene was added at 12 h after removal of hydroxyurea,
possibly on the transition S/G2. Using transmission electron microscopy we observed that the diterpene induced
a delay in the progression of cell division. We conclude that the compound, at cytostatic dose, affects
the cell cycle of T. cruzi, possibly in the transition S/G2 phase and cell division. Further studies will focus to
identify the molecular targets for the action of 5-epi-icetexone.
© 2011 Elsevier Ireland Ltd. All rights reserved
identify the molecular targets for the action of 5-epi-icetexone.
© 2011 Elsevier Ireland Ltd. All rights reserved
identify the molecular targets for the action of 5-epi-icetexone.
© 2011 Elsevier Ireland Ltd. All rights reserved
Here, we studied the effect of the diterpene 5-epi-icetexone on growth and morphology of parasites
synchronized with hydroxyurea, at different periods of time after removal of the nucleotide. We observed
that the diterpene does not affect the growth of the parasites when added within 10 h after removal of hydroxyurea,
but the compound was effective on growth when added to the cultures after 12 h. Thymidine incorporation
was somewhat inhibited when the diterpene was added at 12 h after removal of hydroxyurea,
possibly on the transition S/G2. Using transmission electron microscopy we observed that the diterpene induced
a delay in the progression of cell division. We conclude that the compound, at cytostatic dose, affects
the cell cycle of T. cruzi, possibly in the transition S/G2 phase and cell division. Further studies will focus to
identify the molecular targets for the action of 5-epi-icetexone.
© 2011 Elsevier Ireland Ltd. All rights reserved
identify the molecular targets for the action of 5-epi-icetexone.
© 2011 Elsevier Ireland Ltd. All rights reserved
identify the molecular targets for the action of 5-epi-icetexone.
© 2011 Elsevier Ireland Ltd. All rights reserved
Here, we studied the effect of the diterpene 5-epi-icetexone on growth and morphology of parasites
synchronized with hydroxyurea, at different periods of time after removal of the nucleotide. We observed
that the diterpene does not affect the growth of the parasites when added within 10 h after removal of hydroxyurea,
but the compound was effective on growth when added to the cultures after 12 h. Thymidine incorporation
was somewhat inhibited when the diterpene was added at 12 h after removal of hydroxyurea,
possibly on the transition S/G2. Using transmission electron microscopy we observed that the diterpene induced
a delay in the progression of cell division. We conclude that the compound, at cytostatic dose, affects
the cell cycle of T. cruzi, possibly in the transition S/G2 phase and cell division. Further studies will focus to
identify the molecular targets for the action of 5-epi-icetexone.
© 2011 Elsevier Ireland Ltd. All rights reserved
identify the molecular targets for the action of 5-epi-icetexone.
© 2011 Elsevier Ireland Ltd. All rights reserved
identify the molecular targets for the action of 5-epi-icetexone.
© 2011 Elsevier Ireland Ltd. All rights reserved
Trypanosoma cruzi, the causative agent of Chagas´ disease.
Here, we studied the effect of the diterpene 5-epi-icetexone on growth and morphology of parasites
synchronized with hydroxyurea, at different periods of time after removal of the nucleotide. We observed
that the diterpene does not affect the growth of the parasites when added within 10 h after removal of hydroxyurea,
but the compound was effective on growth when added to the cultures after 12 h. Thymidine incorporation
was somewhat inhibited when the diterpene was added at 12 h after removal of hydroxyurea,
possibly on the transition S/G2. Using transmission electron microscopy we observed that the diterpene induced
a delay in the progression of cell division. We conclude that the compound, at cytostatic dose, affects
the cell cycle of T. cruzi, possibly in the transition S/G2 phase and cell division. Further studies will focus to
identify the molecular targets for the action of 5-epi-icetexone.
© 2011 Elsevier Ireland Ltd. All rights reserved
identify the molecular targets for the action of 5-epi-icetexone.
© 2011 Elsevier Ireland Ltd. All rights reserved
identify the molecular targets for the action of 5-epi-icetexone.
© 2011 Elsevier Ireland Ltd. All rights reserved
T. cruzi, possibly in the transition S/G2 phase and cell division. Further studies will focus to
identify the molecular targets for the action of 5-epi-icetexone.
© 2011 Elsevier Ireland Ltd. All rights reserved