tiRNAs in pulmonary obstructive diseases. Study of their potential role as modulators of corticosteroid response.

MARAVAL, M. B; MINNITI, F; LEDESMA, M; GARDEÑEZ, W; LIOI, S; BOTTASSO, O.A.; SPINELLI, S.V.

Mar del Plata

Congreso; Reunión SAIC SAI SAFIS 2018; 2018

SAIC SAI SAFIS

tiRNAs are 30-40 pb double stranded RNA fragments generated under stress condition by a specific cleavage of mature tRNAs. These molecules have been shown to regulate gene expression by either functioning as novel epigenetic factors or by traslational repression. Different lines of evidence indicate that glucocorticoids (GCs) resistance is the result of significant changes in the cellular microenvironment that occur over time in chronic inflammatory states. Since resistance to corticosteroids in patients with severe asthma and with chronic obstructive pulmonary disease (COPD) is an important barrier to an effective treatment, in this work we studies whether tiRNAs could be playing a role in the corticosteroid response in these diseases. tiRNA-Glu and tiRNA-Gly, two highly abundant tiRNAs in saliva, were successfully quantified in sputum and blood samples (n=20) of healthy individuals and patients with asthma and COPD, both in the intracellular and extracellular fluids. Levels of tiRNA-Glu showed a mild increase in patients with obstructive pulmonary diseases in blood and sputum cells and they positively correlated with cortisol in sputum supernatants (p