Critical lysine demethylase regulation in heart regeneration

NUÑEZ PEDROZO, CRISTIAN; OLEA, FERNANDA D.; PERALTA, TOMÁS; CROTTOGINI AJ; LOCATELLI, PAOLA; CUNIBERTI LUIS

Congreso; Congreso SAFIS-ALACF 2021; 2021

Introduction Heart failure and cardiac injury leads to an irrecoverable loss of cardiomyocytes that significantly reduces heart function and life span in mammalian species. Up to date, the research community has focused on achieving heart regeneration mainly through cardiomyocyte proliferation. Recent publications reveal cardiomyocytes dedifferentiation as the first step in cardiac regeneration. ObjectiveAnalyze the heart expression profile of epigenetics genes related to cell dedifferentiation in a cross-species design.MethodsThe RNA-Seq sample data of each specie was obtained from BioProject database and was further processed on the UseGalaxy platform. The transcriptome analysis was performed employing the EdgeR package with the exact statistical method and the generalized linear model method. The transcriptome collection included the following ones: human (fetal, healthy adult and MI adult samples), sheep (fetal, healthy adult and MI adult samples), mouse (post-natal regenerative window (P1-P7) and non-regenerative (P8-P15)) and lastly, zebrafish (heart samples post cardiac injury (days post injury 1,3,7,14,21)).ResultsThe gene KDM1A had higher expression in the human and sheep fetal condition in comparison to their MI adult counterpart (FDR: