Molecular changes induced by the xenotransplant of human muse cells in the ovine cardic infarction

GIMENEZ S; CROTTOGINI AJ; GIUNTA G; LOCATELLI P; CUNIBERTI L; CASTILLO MG; LÓPEZ AE; OLEA FD

Congreso; European Atherosclerosis Society Congress; 2020

European Atherosclerosis Society

Background and AimsCardiovascular disease (CVD) is the leading cause of morbidity and mortality worldwide, and acute myocardial infarction (AMI) represents the most common CVD. Recently it was shown that human Muse cells (hMuse) are mobilized to the general circulation in patients with AMI and this proved to be a benign predictor of remodeling and ventricular function.Our objective was to study the changes in gene expression involved in differentiation, proliferation and angiogenesis events at seven days of a transplantation with human Muse cells in the infarction area of an sheep model of AMI.MethodsHMuse cells were isolated from human female lipoaspirates using a severe cell stress protocol. A total of 10 million cells were injected at the rate of 10 intramyocardial injections in the peri-infarction zone (hMuse group and placebo group, n = 4). One week later the animals were sacrificed, the tissue samples collected and processed for molecular studies. Finally, capillary and arteriolar densities were measured in representative samples of myocardium by Immunohistochemistry.ResultsThe expression of gata4 and nkx2.5 was higher in the hMuse group compared to placebo.Arterial and capillary density was higher in the hMuse group compared to placebo (21.3±2.1vs.10.4±1.0 arterioles/mm2, p