IMMUNOHISTOCHEMICAL ANALYSIS OF SKIN BIOPSIES

VIGLIANO C; MAZZUOCCOLO L; BERTOCCHI G; ALVAREZ MG; SEIGELFISCHER D; LOCOCO B; VIOTTI R; LAUCELLA S; ALBAREDA MC; FERNáNDEZ M; CASTRO EIRO, MD

Buenos Aires

Congreso; Reunión conjunta de Sociedades de Biociencias; 2017

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA

Benznidazole is one of the two drugs available for treatmentof Chagas disease with a suspension rate of approximately 17-30% in chronically T. cruzi-infected adult patients primarily due to theappearance of dermatitis. We have previously shown that some ofthese patients have IFN-γ producing T cells reactive to benznidazole.The aim of this study was to analyze the phenotype and functionof cells present in skin lesions of patients treated with benznidazolewho presented dermatitis. Four skin biopsies were preserved in formaldehyde10 %. Tissue samples were then dehydrated, cleared,embedded in paraffin, cut into 4?5 μm thick sections and stainedwith Hematoxylin and Eosin. Immunohistochemistry was performedusing specific antibodies for human CD4, CD8, perforin, IL5and IFN-γ molecules. All of the biopsies analyzed presented inflammatoryinfiltrate with eosinophilia. CD8 T lymphocytes but not CD4were found with this technique. Secretion of perforin was observedin all cases whereas IL-5+ and IFN-γ+ cells were not found. Thesefindings strengthen the evidence supporting that adverse reactionsto benznidazole are cell mediated with the involvement of CD8+ Tcells.