Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis

CHEIJ, ESTEBAN; CÉSPEDEZ, JORGE; OLEA, GABRIELA; RODRIGUEZ, FLORENCIA; BOCCIONI, ANA PAULA C.; LOMBARDO, DANIEL

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS.

ACAD BRASILEIRA DE CIENCIAS

Lugar: Rio de Janeiro; Año: 2021 vol. 93 p. 1 - 11

0001-3765

To characterize the reproductive biology of Scinax acuminatus and contributeto the natural history of this species, the morphology of the reproductive system ofmales and females was analyzed at anatomical, histological and immunohistochemicallevels. The individuals were collected fortnightly between August and December(2016) and January to December (2018). The anatomy of the reproductive system wasanalyzed in a stereoscopic microscope, and histological preparations staining withhematoxylin-eosin and Masson?s trichromic, PAS and Coomassie Blue was performedas well. To characterize the meiotic-active cells in the testes, immunostaining with thePCNA proliferation protein was performed. There were found females with ovaries withoocytes in different stages of maturity and post-ovulatory females. Males presentedcontinuous spermatogenesis, which could be confirmed by the immunostaining of PCNAin spermatogonia during the cycle. The results of this work serve as a basis for thecharacterization of the reproductive cycle in S. acuminatus and provide backgroundinformation on the analysis of spermatogenic activity by IHQ from the study of theimmunodetection of the PCNA cell proliferation protein. Future studies will focus on theevaluation of cell death processes during the reproductive cycle in the studied speciesto compare with those obtained in terms of cell proliferation.