Role of FSH glycan structure in the regulation of Sertoli cell inhibin production

ANDREONE LUZ; AMBAO VERÓNICA; PELLIZZARI ELIANA; LORETI NAZARETH; CIGORRAGA SELVA; CAMPO STELLA

REPRODUCTION

BIOSCIENTIFICA LTD

Lugar: Bristol; Año: 2017

1470-1626

Variations in FSH carbohydrate composition and structure are associatedwith important structural and functional changes in Sertoli cells (SCs) duringsexual maturation. The aim of the present study was to investigate the impactof FSH oligosaccharide structure and its interaction with gonadal factors onthe regulation of monomeric and dimeric inhibin production at differentmaturation stages of the Sertoli cell. Recombinant human FSH (rhFSH)glycosylation variants were isolated according to their sialylation degree (AC and BA) and complexity of oligosaccharides (CO and HY). Native rhFSHstimulated inhibin α-subunit (Pro-αC)but did not show any effect on inhibin B (InhB) production in immature SCsisolated from 8-day-old rats. Activin A stimulated InhB and had a synergisticeffect on FSH to stimulate Pro-αC. The less acidic/sialylated rhFSH charge analogues, BA, were the only charge analogue mix that stimulated InhB aswell as the most potent stimulusfor Pro-αC production. Native rhFSH stimulated bothPro-αC and InhB in SCs at a more advanced maturation stage, isolated from20-day-old rats. In these cells, all rhFSH glycosylation variants increasedInhB and Pro-αC production, even in the presence of growth factors. The BA preparationexerted a more marked stimulatory effect on InhB and Pro-αCthan the AC. Glycoforms bearing high mannose and hybrid-type oligosaccharides,HY, stimulated InhB and Pro-αC more effectively than those bearingcomplex oligosaccharides, CO, even in the presence of gonadal growth factors.These findings demonstrate the modulatory effect of FSH oligosaccharide structureon the regulation of inhibin production in the male gonad.