The Rhinella arenarum transcriptomics: assembly, annotation and gene prediction in organophosphate exposure.

LASCANO, CECILIA INES; CESCHIN, DANILO GUILLERMO; MARDIROSIAN, MARIANA NOELIA; PIRES, NATALIA SUSANA; VENTURINO, ANDRES

Congreso; Brazilian-International Congress of Genetics; 2017

Northern Patagonia is an irrigated area holding 95% of exportable production of apple and pear of Argentina. The assessment of the impact of pesticides and other environmental toxicants generated by anthropic action requires the inclusion of effects studies on native species. It is known that there are biochemical and molecular responses that manifest in concentration ranges of a toxicant well below to those that cause physiological alterations and lethal effects. Determining what the mechanisms of action and response may be in sensitive and early biomarkers of toxic exposure is needed. Biomonitoring of environmental impact of pesticides using native species is a preferred resource for its ecological significance. However, it proves to be an arduous task in the absence of knowledge, for instance, of their genomes. Obtain the genome sequence is still very expensive but a transcriptomic approach using Next Generation Sequencing (RNA-Seq) is affordable and make available a huge set of information on gene expression. Rhinella arenarum is a toad widely distributed in Argentina and less in Brazil, Bolivia and Uruguay. This amphibian can be used in ecotoxicological research and environmental toxicology.