IICAR 25568
INSTITUTO DE INVESTIGACIONES EN CIENCIAS AGRARIAS DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Target-site resistance to ALS-inhibiting herbicides in Amaranthus palmeri from Argentina
Autor/es:
LARRAN ALVARO; LIEBER LUCAS; PEROTTI VALERIA; PERMINGEAT HUGO; PALMIERI VALERIA; TUESCA DANIEL
Revista:
PEST MANAGEMENT SCIENCE
Editorial:
JOHN WILEY & SONS LTD
Referencias:
Lugar: Londres; Año: 2017 vol. 73 p. 2578 - 2584
ISSN:
1526-498X
Resumen:
BACKGROUND: Herbicide resistant weeds are a serious problem worldwide. Recently, two populations of Amaranthus palmeri with suspected cross-resistance to ALS-inhibiting herbicides (R1 and R2) were found by farmers in two locations from Argentina (Vicuña Mackenna and Totoras, respectively). We conducted studies to confirm and elucidate the mechanism of resistance.RESULTS: We performed in vivo dose-response assays, and confirmed both populations had a strong resistance to chlorimuron-ethyl, diclosulam and imazethapyr when compared to a susceptible population (S). In vitro ALS activity inhibition tests only indicated a considerable resistance to imazethapyr and chlorimuron-ethyl, indicating that other non-target mechanisms could be involved in diclosulam resistance. Subsequently, molecular analysis of als nucleotide sequences revealed three single base-pair mutations conferring substitutions in amino acids previously associated with resistance to ALS inhibitors, A122, W574, and S653CONCLUSION: This is the first report of als resistant alleles in Amaranthus palmeri from Argentina. The data support the involvement of a target-site mechanism of resistance to ALS inhibiting herbicides.
