HYPOPHYSITIS IN NON-OBESE DIABETIC MICE AS A NEW TISSUE- SPECIFIC AUTIMMUNE RESPONSE

MORENO-SOSA MT; SOAJE M; SANCHEZ B; YÚDICA SEDANO F; NEIRA F; PENNACCHIO GE; PIETROBON EO; JAHN GA; MACKERN-OBERTI JP

Buenos Aires

Congreso; 62 Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica.; 2017

SAIC

Type 1 diabetes mellitus is a chronic organ-specific autoimmunedisease that results from the destruction of beta (β) cells in the pancreaticislets and shows a pronounced leukocyte infiltration in thegland. Non-obese diabetic (NOD) mice develop autoimmune diabetesapproximately at 6 months of age in females, providing anexperimental model for human Type 1 Diabetes. They also displayadditional autoreactive immune responses against other glands includingthe prostate and thyroid. Furthermore, female NOD miceexhibit a lower fertility index compared to wild type mice, that developsapproximately at the same time as diabetes. To determinewhether the low fertility of NOD mice is caused by an autoimmuneresponse against the hypophysis, we assessed the presence of leukocyteinfiltration in the gland. To this end, the presence of CD45+(panleukocyte) cells in the hypophysis of 3 and 6 months old femaleBALB/c (control group) and NOD mice was analyzed by flow cytometry.An increase in leukocyte infiltration in the hypophysis from6 months old NOD mice was observed compared to the controls(0.05±0.04 % BALB/c mice vs 3.2±1.0% NOD mice; p