Long-term effects of maternal mid hyperthyroidism on the development and the behavior of offspring in adulthood.

MITCHEL MC; SANCHEZ MB; NEIRA FJ; VIRUEL, L.B.; SOAJE, M.; PIETROBON, E.O.; GOMEZ S; JAHN, G.A.; MACKERN-OBERTI JP

Mendoza

Congreso; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo.; 2022

Sociedad de Biología de Cuyo

Abnormal levels of thyroid hormones (THs) are associated with alterations in anxiety and circulating hormones such as glucocorticoids(GCs) and prolactin affecting fertility and reproductive success. However, little is known about the long-term effects of maternalhyperthyroidism on the development and behavior of their offspring. For this purpose, eight pregnant Wistar rats were divided in twogroups defined as: Control (Euthyroid, n=4) and HyperT (n=4). Hyperthyroidism was induced with T4 0.1mg/kg/day, s.c. After delivery,on day 2 of lactation, the number of pups in each litter was standardized to eight and the offspring allowed to growth under standardcondition. Development parameters were recorded from birth to 42 postnatal day (PND). Adult female and male offspring (PND 85-100)were subjected to the Open Field Test (OFT) to evaluate locomotor activity using EthoWatcher computacional tool. The parametersassessed were as follows: a) rearings, b) entries to areas of interest, c) total distance traveled. At PND 100-120, the adult offspring weresacrificed, trunk blood was collected for serum hormonal determinations and adrenal glands (AG) dissected for histological analysis usingImage J program. We found that offspring from HyperT mothers (named HyperT pups) presented alterations in the postnatal developmentof some physical, sensory, motor, and reproductive parameters compared to offspring of euthyroid mothers (named Control pups). Bodylength at PND 1 was lower in HyperT pups (p