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SOAJE Marta
congresos y reuniones científicas
Título:
Desmoglein-4 deficiency weakens ova specific IgA and IgM humoral immunity.
Autor/es:
VIRUEL, L.B.; SANCHEZ MB; MITCHEL MC; TRONCOSO M; NEIRA FJ; CAMPOVERDE ARBOCCO F; SOAJE, M.; SANCHEZ MV; LUCERO F; TRONCOSO A; MOISO A; PIETROBON, E.O.; INNOCENTI BADANO AC; JAHN, G.A.; VALDEZ SR; MACKERN-OBERTI JP
Lugar:
Mendoza
Reunión:
Congreso; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo.; 2022
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
Desmogleins (Dsg) are cadherin-type proteins involved in the adhesion mechanisms carried out by desmosomes. Dsg-4 deficiency is associated with hair follicle alterations and hair loss in humans, mice, and rats. Recently, we have reported that the imiquimod topic administration to Dsg-4 deficient rats leads to an exacerbated skin inflammation compared to healthy control animals. Unfortunately, the role of Dsg-4 in IgA and IgM humoral immunity has not been addressed. The focus of our work was to determine whether desmoglein 4 deficiency impairs OVA-specific IgA and IgM induction. For this purpose, Desmoglein 4 deficient rats OFA hr/hr (Dsg4 null) and wild-type Sprague Dawley (SD) rats were inoculated intradermal with 20μg/40μl OVA/PBS. Two weeks later, serum samples were obtained to determine OVA-specific IgA and IgM levels by ELISA. Surprisingly, Dsg4 null rats displayed lower OVA-specific IgM (OD at 1/100; Dsg4 null 0.152±0.053 vs SD 0.427±0.194, t test, p=0.0383) and IgA levels compared to SD group (OD at 1/100; Dsg4 null 0.094±0.021 vs SD 0.1736±0.025, t test, p=0.0383). When we evaluated axillar lymphatic nodes expansion after topic imiquimod administration for 3 consecutive days every week for 2 weeks, we found that Dsg4 null rats displayed a lymphatic node weight increased compared to SD rats (imiquimod Dsg4 null 375mg ± 27 vs imiquimod SD 185mg ± 25, t test, p=0.0043; untreated Dsg 4 null 58mg ±3 and SD 43 mg ±6). These results suggest that desmoglein-4 may help in mounting specific humoral immunity. Several mechanisms may be involved including antigen lymphatic drainage, keratinocyte-derived cytokines, aberrant crosstalk between keratinocyte and dendritic cells, leading to an impaired germinal center reaction. Although further research is needed, our results assign a new role to Dsg-4 in inducing humoral immunity during an intradermal antigen exposure.AREA TEMÁTICA5) Microbiología e Inmunología (MI)