Characterization of nanoparticles for vaccines development

RIZZO, GASTÓN; AGAZZI MAXIMILIANO; DOCENA GUILLERMO H.; HERRERA SANTIAGO; AZZARONI OMAR; SMALDINI, PAOLA LORENA; CORTEZ LORENA

Congreso; LVII REUNIÓN ANUAL SAI 2019; 2019

Nanotechnology are becoming to play an important role in vaccine development, it offers the opportunity to design nanoparticles varying in composition, size, shape and surface properties for application in the fields of medicine. In this work, we aimed to characterize nanoparticles as a safety adjuvant for further use in immunotherapies. Polycationic polymer nanoparticles (R3N0) were used in BMDC to characterize them in vitro. Nanoparticle-cell interaction (internalization or adhesion) was evaluated by microscopy and cell phenotype activation (MHCII and CD86) were determined by flow-cytometry. IL-1β-production was also evaluated by ELISA. Furthermore, Balb/c mice were intraperitoneally sensitized with R3N0, R3N0 plus OVA (combined) and OVA conjugated to R3N0. Finally, humoral (serum antibodies) and cellular immune response (cytokines) was evaluated by ELISA and flow cytometry. We found that R3N0 is internalized by the cells generating its activation with an increase in the expression of CD86 and IL-1β. In turn, R3N0 promotes grater stimulation and release of IL-1β than the positive control (P