Arachidonic acid modulation of BK (Slo1) channels: Role of the beta1 accessory subunit.

MARTIN, P; ORSI, F; MILESI, V; MONCADA, M; CASTILLO, K; DUCCA, G; GONZÁLEZ, C

Valparaíso

Congreso; Society of General Physiologists (SGP) 73rd Annual Symposium Sociedad de Biofísicos Latinoamericanos (SOBLA) Annual Meeting; 2019

Society of General Physiologists (SGP) y Sociedad de Biofísicos Latinoamericanos (SOBLA)

Arachidonic acid (AA) is a fatty acid involved in modulation of several ion channels. Previously, wereported that AA activates the high conductance Ca 2+ - and voltage-dependent K + channel (BK) invascular smooth muscle cells where the channel is expressed with the accessory β1-subunit (β1)[Martín et al. 2014. Pflugers Arch. 466(9):1779-92]. Here, we studied in depth the actionmechanism of AA using the patch-clamp technique on BK channel heterologously expressed withβ1. 10 µM AA activated the BK channel by a left shift on G-V curve (ΔV 1/2 = -55.2 mV ± 4,4; n=3;p