HEK-BLUE TLR4 REPORTER CELL LINE POTENTIAL TO DISTINGUISH FUNCTIONAL CHANGES IN STOOL'S MICROBIOTA

ERREA A; ROLNY I; GIRARDI JI; RUMBO M

San Miguel de Tucumán

Congreso; LXVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA; 2019

Sociedad Argentina de Inmunología

Ambiental factors may condition the composition of intestinal microbiota with importantimpact in a wide variety of diseases, including type 2 diabetes, obesity, cancer, Crohn?s diseaseand even neurodegenerative diseases. Different antibiotic treatments may have differentialimpact on microbiota functional properties.The aim of our work is to characterize the potential of a HEK-Blue? hTLR4 reporter cell line toassert changes in intestinal microbiota from stool in an antibiotic-induced dysbiosis model inmice. We treated mice with ampicillin, vancomycin or azithromycin and compared stool?s TLR-4agonist activity and microbiota composition assessing stool?s DNA by qPCR. Non-treated micewere used as controls.We observed a differential stimulatory activity of hTLR-4 reporter cell line depending on theantibiotic treatment. While stools from azithromycin-treated mice showed lower TLR-4 agonistactivity than controls, vancomycin and ampicillin promoted an increase in stimulatory potential(p< 0.0001). Microbiota analysis showed no changes in Firmicutes, a decrease on Bacteroidetesand a decreasing tendency of Proteobacteria population for azithromycin. Vancomycin andampicillin, although both increased hTLR-4 agonist activity in stool, showed different changes inmicrobiota. Both vancomycin and ampicillin induced a relative decrease in Bacteroidetes(p