HEK-BLUE TLR4 reporter cell line potential t odistinguish functional changes in stool´s microbiota

ERREA A.; GIRARDI J.I.; RUMBO M; ROLNY I.

Tucuman

Congreso; LXVII Reunion Cientifica Anual de la Sociedad Argentina de Inmunología; 2019

SAI

Ambiental factors may condition the composition of intestinal microbiota with important impact in a wide variety of diseases, including type 2 diabetes, obesity, cancer, Crohn?s disease and even neurodegenerative diseases. Different antibiotic treatments may have differential impact onmicrobiota functional properties.The aim of our work is to characterize the potential of a HEK-Blue? hTLR4 reporter cell line to assert changes in intestinal microbiota from stool in an antibiotic-induced dysbiosis model in mice.We treated mice with ampicillin, vancomycin or azithromycin and compared stool?s TLR-4 agonist activity and microbiota composition assessing stool?s DNA by qPCR. Non-treated mice were used as controls.We observed a differential stimulatory activity of hTLR-4 reporter cell line depending on the antibiotic treatment. While stools from azithromycin-treated mice showed lower TLR-4 agonist activity than controls, vancomycin and ampicillin promoted an increase in stimulatory potential (p< 0.0001). Microbiota analysis showed no changes in Firmicutes, a decrease on Bacteroidetes and a decreasing tendency of Proteobacteria population for azithromycin. Vancomycin and ampicillin,although both increased hTLR-4 agonist activity in stool, showed different changes in microbiota.Both vancomycin and ampicillin induced a relative decrease in Bacteroidetes (p