OVERLAPPING CELL DEATH PATHWAYS IN HUMAN SMALL INTESTINE IN ACTIVE ENTEROPATHY

PEREZ, FEDERICO; MENENDEZ, LORENA ; RUERA, CAROLINA N; GUZMAN, LUCIANA; CHIRDO, FERNANDO; BACCIADONE, RODRIGO; GARBI, LAURA

Tucuman

Congreso; LXVII Reunión científica anual de la Sociedad argentina de inmunología; 2019

Sociedad Argentina de Inmunología

Celiac disease (CD) is a chronic enteropathy characterized by severe changes in the histology of the proximal small intestine, among them crypt hyperplasia and loss of villi, accompanied by death of epithelial cells. Though traditionally, apoptosis has been pointed out as the main deathpathway, mechanisms of cell death were not completely established. The aim of this study is to get a deeper knowledge of the cell death pathways in human small intestinal mucosa.Duodenal biopsies were collected from pediatric and adult patients during the routine procedure for CD diagnosis. Evaluations were performed on sections of paraffin-embedded tissues and western blot (WB) analysis in proteins extracts from whole biopsy sample.By confocal fluorescence microscopy (IFI), TUNEL reaction showed increased number of death cells in lamina propria of CD patients compared with healthy controls (p< 0,002). These TUNEL+ cells express CD45, meaning that they are bone marrow derived and not stromal cells.TUNEL+ cells were composed by, approximately, 75% CD138+, 20% CD20+ and 5% CD3+ cells.The assessment of death pathways by IFI analysis showed significative increment in caspase 3 and 8 of duodenum of CD patients. WB analysis confirmed that active caspase 3 is increased (p