Analysis of the eosinophil-chemoattractant cytokine production in intestinal tissues of patients with food allergy

CANZIANI KARINA; ALATAMIRANO M EUGENIA; GONZALEZ VILLAR M TERESA; DOCENA GUILLERMO; ROLNY IVANNA; BERNEDO VIVIANA; MUGLIA CECILIA; VACCARO JULIAN; GUZMAN LUCIANA; GARCIA MARCELA

MAR DEL PLATA

Congreso; Congreso Conjunto SAIC-SAI-SAFIS; 2018

Juvenile polyps (JP) are common in pediatric patients with milk allergy and lead to rectal bleeding and diarrhea. We have previously characterized the allergic inflammatory cell infiltrate of the polyp tissue, and here we aimed to investigate the origin of the high frequency of eosinophils, which may be responsible for tissue remodeling. Biological intestinal samples were obtained by colonoscopy in 7 patients, along with a biopsy of the surrounding tissue (SCT), as a control sample. The production of eotaxin-3 (CCL26) and RANTES, chemokines responsible for eosinophil homing, were assessed by qPCR and confocal microscopy in lamina propria and the epithelial compartment. In addition, Caco-2 and HMEC-1 cell lines were employed to study the induction of chemokine production. Cells were stimulated with IFN-γ (10ng/ml) and IL-13 (10ng/ml), and the activation of STAT-3 and expression of chemokines were analyzed by immunoblotting and qPCR, respectively. We found a significant increase of eosinophils and eotaxin-3 expression in polyps compared to SCT (p