Local immunoglobulin E production in colorectal polyps of patients with food allergy.

PUCCI MOLINERIS MELISA; NANFITO GABRIELA; ALTAMIRANO MARÍA EUGENIA; MUGLIA CECILIA; CANZIANI KARINA; DIAZ JIMENEZ DAVID; GARCIA MARCELA; RAMELLO HERMOSO MARCELA; BALCARCE NORMA; GUZMAN LUCIANA; MEIER DOMINIK; DOCENA GUILLERMO

SAN FRANCISCO

Congreso; Federation of Clinical Immunology Societies Congress; 2018

Juvenile polyps (JP) are common in children. The aetiology of JP remains largely unknown and few reports have characterized its stroma in atopic patients. We previously showed that 70 % of patients older than 1 yo, with rectal bleeding and JP had high levels of serum IgE specific to cow milk proteins (CMP). This finding prompted us to study the relation between the aetiology of polyps and the atopic condition of patients. Resected polyps (PT) and surrounding mucosa (SCT) were studied (n=10) by histopathology (H&E), confocal microscopy and RT-qPCR; cytokines were assessed by CBA and ELISA; germinal centers were isolated by dissection laser microscopy and analyzed by PCR. We found in 7 patients with serum CMP-specific IgE a cell infiltrate in the stroma of polyps dominated with eosinophils, mast cells, IgE-producing plasmatic cells , ST2+ , TSLP+ and IL33+ cells. SCT showed fewer frequencies of IgE+ cells (30±10 cells vs 2±1 polyps vs mucosa). Furthermore, we found higher transcription levelsof il-4, il-5, il-33, ST2s (p