CHARACTERIZATION OF THE TSLP AND IL-33 AXIS IN THE INFLAMMATORY COLONIC TISSUE OF FOOD ALLERGIC PATIENTS WITH JUVENILE POLYPS

HERMOSO RAMALLO MARCELA; GARCIA MARCELA; GUZMAN LUCIANA; DOCENA G; CUETO RUA EDUARDO; MUGLIA CECILIA; DIAZ-JIMENEZ DAVID; ALTAMIRANO EUGENIA; ALCALDE MB; BALCARCE NORMA; CANZIANI KARINA

CABA

Congreso; Reunión Conjunta de Sociedades de Biociencia, LXV Reunión Annual de la Sociedad Argentina de Inmunología; 2017

diarrhea. The aetiology is largely unknown and we have previously characterized the allergic inflammatory infiltrate of the polyp tissue in children with clinical suspicion of milk allergy. We found high frequency of eosinophils, IgE-producing plasma cells, Th2 cells, high level of type 2 cytokines and augmented expression of ST2 and IL33. These results lead us to investigate the production of thymic stromal lymphopoietin (TSLP) and IL-33 as contributors to the development of the Th2 local immune response. TSLP and IL-33 expression were assessed in the epithelial compartment and lamina propria of colonic polyps (P) and surrounding colonic tissues (SCT). Biological samples were obtained in 7 patients by colonoscopy and analysis were performed by confocal microscopy and qPCR. We found a significant increased expression of IL-33 in epithelial cells from P compared with SCT (p