Proinflammatory response of human endometrial epithelial cells to Brucella abortus infection.

ALONSO PAIVA IM; FERNANDEZ AG; FOSSATI, CA; RITTER A; BALDI PC,; MUÑOZ GONZÁLEZ F,; FERRERO MC,

Buenos Aires

Congreso; II Reunión Conjunta de Sociedades de Biociencias y LXV Reunión Anual de la Sociedad Argentina de Inmunología; 2017

Brucella induces abortion in humans and animals. Therefore, the interaction between uterine cells and Brucella is important for understanding the pathogenesis of this disease. In this work, we evaluate the inflammatory response (chemokine and metalloproteinases) and the expression of hormonal receptors and various microRNA related to infection or inflammation processes in the human endometrial epithelial cell line Ishikawa, after infection with B. abortus 2308 or stimulation with conditioned medium (CM) from infected human phagocytes or trophoblasts. The Ishikawa cells, the monocytic cell line THP-1, the trophoblastic cell line Swan-71 and monocytes and neutrophils isolated from peripheral blood were infected with B. abortus for 2 h. After treatment with gentamicin, supernatants were collected at 24 and/or 48 h. Here we show that B. abortus invades and replicates in Ishikawa cells without inducing an increase in interleukin 8 (IL-8) or monocyte chemoattractant protein 1 (MCP-1) secretion. However, the stimulation of Ishikawa cells for 48 h with CM from B. abortus-infected THP-1 cells, primary monocytes orSwan-71 cells induces a significant increase of IL-8 and MCP-1 as compared to stimulation with CM from non-infected cells (p