Pro-inflammatory effect of volvanic ash (v.ash) ina a colitis model- is the v.ash or some of its components linked with the IBD epidemiology?

ORSINI DELGADO L; SAMBUELLI A; PAPA GOBBI R; GIL A; NEGREIRA S; GONCALVES S; HUERNOS P; BELLICOSO M; TIRADO P; CHAVERO P; DOCENA G

Barcelona

Congreso; 10th European Crohn´s and colitis organization; 2015

BackgroundIt is widely accepted a role for environmental factors in the IBD pathogenesis. Urban pollutants were associated with hospitalization rates and early IBD debut. Intestinal exposure to particulate matter (PM) has proinflammatory effect (instilled in mice) inducing permeability and TNFα. Volcanic pollution, implicated in varied health impairments, was not studied in IBD. Increase PM remained after Iceland eruptions by ash resuspension(Thorsteinsson T,2012). High IBD incidences were reported from Iceland (volcanic) Faroe Islands, N.Zealand. During devastating Peyehue (Patagonian volcano,2011) eruptions, PM increase by ash-carrying winds were detected in Buenos Aires (Argentina), as well refractoriness in Hospitalized IBD of a reference centre. Hypothesis: volcanic ash (V.ASH) may be pro-inflammatory in IBD. Aim: to study the effect of V.ASH (from Patagonia) in a colitis mouse modelMethodsBALB/c mice received drinking water with or without V.Ash for 14 days. On day 7 different inflammatory stimuli were intrarectally administered: TNBS, Flagellin (FliC) or ethanol (EtOH) as vehicle control. On day 14 mice were sacrificed and the colon studied (weight, length, histology, ZO-1 immunofluorescence, gene expression of IL1 β, IL6, TNFα, Ccl20, IFN γ, Tbet, IL17A, RORγt by qPCRResultsWe found a marked weight loss in mice receiving drinking water+ash vs mice with only drinking water or EtOH. Colon of mice receiving ash showed a greater ratio weight/length EtOH/EtOH+ash 28.4 ± 4.4 vs 31.3 ± 2.4.0 TNBS/TNBS+ash 28.3 ± 0.1 vs 33.7 ± 4.2, FliC/FliC+ash 24.7 ± 4.01 vs 31.5 ± 5.9). Histological activity index: EtOH/EtOH+ash: 1 vs 3 (p