BENEFICIAL EFFECTS OF PROBIOTIC ADMINISTRATION IN AN EXPERIMENTAL MODEL OF SMALL INTESTINE INFLAMMATION

CAROLINA N. RUERA; IRIBARREN MARÍA LUZ; DUCCA, GERÓNIMO; CARASI, PAULA; MICULÁN, EMANUEL; FEDERICO PEREZ; CHIRDO FERNANDO

Mar del plata

Congreso; LXX Reunión anual de la sociedad argentina de inmunología; 2022

Sociedad argentina de inmunología

Several animal models have shown the beneficial effect of probiotics in the control of inflammation in the colon. However, information about the role of probiotics in small intestine is limited. Theaim of our work is to evaluate the effect of precolonization with two probiotic strains (Lactobacillus kefiri and Enterococcus durans), in a previously characterised murine model of enteropathy induced by a gliadin-derived peptide (p31-43). Material-Methods. C57BL/6 wild type mice were treated by intragastric administration (IG) with (108 CFU) L. kefiri or E. durans, 3 times a week, for 3 weeks, or vehicle. Then, mice were treated by IG with p31-43 (20µg/mouse) or vehicle. After 16 hs, small intestines were collected. Sections from proximal small intestine were H&E stained for Villus height /Crypt depth ratio (V/C) analysis and counting of intraepithelial lymphocytes (IELs). TUNEL reaction was used to evaluate cell death. Protein extracts were used for western blot (WB) analysis. Results. P31-43-treated mice showed a decrease in V/C ratio and increased number of IELs, compared to control groups. Treatment with both probiotics inhibited the tissue damage upon p31-43 challenge. Similarly, TUNEL reaction showed a reduction on the number of dead cells in mice receiving L. kefiri or E. durans and p31-43 challenge, compared with mice treated with p31-43 alone (p< 0,001). WB analysis showed a decrease in active caspase-1/procaspase-1 ratio in small intestine of C57BL/6 mice treated with any of both probiotics and p31-43 peptide challenge compared with p31-43-treated mice (p