Effects of developmental exposure to glyphosate and propiconazole on the male rat mammary gland

GOMEZ AYELEN L; MUÑOZ-DE-TORO MONICA; ALTAMIRANO GABRIELA A; KASS LAURA; SCHIERANO MAROTTI GONZALO

Modalidad Virtual

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020; 2020

Sociedad Argentina de Investigación Clínica, Sociedad Argentina de Inmunología, Sociedad Argentina de Fisiología

Several agrochemicals have been shown to impaired mammarygland development, some of them acting as endocrine disruptingchemicals. Since the male rat mammary gland is susceptible toendocrine disruption, it is a useful model to study the effects of thesecompounds. Here, we evaluated whether developmental exposureto glyphosate (GLY), propiconazole (PRO) or both (PROGLY) alter the mammary gland morphology and its endocrine response inpostpubertal male rats. Pregnant rats were exposed orally to vehicle(saline solution), 4 mg/kg/day of GLY, 4 mg/kg/day of PRO or bothdoses of GLY and PRO, from gestation day 9 until weaning. On postnatal day 60, the male offspring were sacrificed and mammary glandsamples were collected. Total area, perimeter, longitudinal growth,number of terminal end buds and mammary development scorewere analyzed in mammary gland whole-mounts (WMs). Lobuloalveolar development and the presence of hyperplastic structureswere evaluated in histological sections, as well as estrogen (ESR1)and androgen receptor (AR) protein expression. Among the parameters evaluated in WM, only mammary gland total area was reducedin GLY exposed males (Control: 492±16 mm2, GLY: 419±21 mm2,PRO: 436±17 mm2, PROGLY: 473±19 mm2; Control vs GLY p