Early postnatal exposure to xenoestrogens alters the expression of molecules involved in the postnatal differentiation of the oviduct of the Broad-Snouted Caiman (Caiman latirostris)

MORA, S.C.; TAVALIERI, Y.E.; LUQUE, E.H.; GALOPPO, G.H.; MUÑOZ DE TORO, M.M.

Mar del Plata

Congreso; XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); 2019

Sociedad Argentina de Biología

The differentiation of the oviduct of Caiman latirostris is completed postnatally and is characterized by temporal-spatial patterns of histofunctional changes. We have shown that the exposure to Bisphenol A (BPA) or 17β-Estradiol (E2) at critical stages of embryo development affects oviduct postnatal differentiation, leading to a precocious adenogenesis. Adenogenesis is regulated by the Wnt signaling pathway in many vertebrates. The aim of this study was to determine the molecular mechanism behind altered adenogenesis. We established the ontogeny and evaluated the effects of postnatal exposure to BPA or E2 on the expression of molecules of the Wnt signaling pathway: Wnt-7a, Wnt-5a, β-catenin and FoxA2. Archived paraffin-embedded samples were used to establish ontogenies. To assess the effect of xenoestrogens, early postnatal caimans were treated with E2 (0.014 or 1.4 ppm) or BPA (1.4 or 140 ppm). Protein expression was assessed by IHC. ANOVA or Kruskal-Wallis was performed to obtain the overall significance, followed by Dunnett?s or Dunn?s test, respectively; p