Endosulfan alters myometrial adaptation during early pregnancy and labor in neonatally exposed rats.

ALARCÓN R; CADAVIZ FERNÁNDEZ DB; MILESI, MM; PACINI G; VARAYOUD J.; LORENZ V; LUQUE EH

Simposio; International Federation of Placenta Associations 2019 (IFPA2019) - 8th Latin American Symposium on Maternal-Fetal Interaction and Placenta (VIII SLIMP).; 2019

Proper myometrial adaptation during gestation is crucial for embryo implantation, pregnancy maintenance and parturition. Abnormal myometrial differentiation or contractility might promote several reproductive disorders such as infertility, implantation failure or preterm birth. Previously, we reported that neonatal exposure to endosulfan alters uterine development and induces implantation failures. The present work investigates the effects of endosulfan exposure on myometrial differentiation at the pre-implantation period, and myometrial activation during labor. Newborn female rats were s.c. injected with corn oil (vehicle) or 600 µg/kg/day of endosulfan (Endo600) on postnatal days (PND) 1, 3, 5 and 7. On PND90, the rats were mated to evaluate: i) the myometrial differentiation on gestational day 5 (GD5, pre-implantation period), by assessment myometrial histomorphology, smooth muscle cells proliferation, and expression of proteins involved in myometrial adaptation for embryo implantation (steroid receptors, Wnt7a and Hoxa10); ii) the timing of parturition and myometrial activation during labor by determining the uterine expression of contraction-associated genes (oxytocin receptor, OTXR; prostaglandin F2α receptor, PTGFR and connexin-43, Cx-43). Endosulfan decreased the thickness of both myometrial layers, with a concomitant decrease in the collagen remodeling. Blood vessels relative area in the interstitial connective tissue between muscle layers was also decreased. Endo600 group showed lower myometrial proliferation in association with a downregulation of Wnt7a and Hoxa10. Even though labor occurred on GD23 in both experimental groups, endosulfan altered the timing of parturition by inducing advancement in the initiation of labor. This alteration was associated with an increased uterine expression of OTXR, PTGFR and Cx-43. In conclusion, neonatal exposure to endosulfan produced long-term effects affecting myometrial adaptation during early pregnancy and labor. These alterations could be associated with the aberrant effects of endosulfan on the implantation process and the timing of parturition.