DISTURBANCE OF PLACENTAL AND FETAL RAT DEVELOPMENT CAUSED BY CAFETERIA DIET

VARAYOUD J; RAMOS JG; ROSSETTI MF; VARAYOUD J; RAMOS JG; LUQUE EH; ZIERAU O; ROSSETTI MF; SCHUMACHER R; STOKER C; GASTIAZORO MP; GUERRERO SCHIMPF ML; LUQUE EH; ZIERAU O; SCHUMACHER R; STOKER C; GASTIAZORO MP; GUERRERO SCHIMPF ML

Berlín

Encuentro; Annual Young Active Research in Endocrynology (YARE) Meeting; 2017

German Society of Endocrinology

Obesity is increasingly common in Western societiesand is associated with fetal and placental growth restriction. The mechanismunderlying the fetal and placental growth restriction is unknown. In addition,estrogens, through estrogen receptors (ERs) affect placental and fetaldevelopment. The aim was evaluate the cafeteria diet (CAF) effects on: 1)reproductive performance and serum levels of Estradiol and Progesteronehormones, 2) fetal and placental growth on gestational day 21 (GD21), 3) weightof pups at birth and 4) expression of ER alpha (ERa) mRNA in rat placentas onDG21. Twenty-one-day-old female Wistar rats were fed after weaning with astandard rodent chow diet (control) or cafeteria diet (CAF) with highlypalatable energy dense foods. On postnatal day 90, rats were mated toevaluate the reproductive performance, placental and fetal weight, andplacental-fetal weight ratio. Total RNA from the frozen placenta samples(GD21) was extracted and subjected to real-time RT-PCR analysis of ERatranscripts. The CAF diet did not alter reproductive performance,serum levels of hormones and fetal weights, however CAF group showed a decreaseplacental weight, placental-fetal weight ratio, and weight of pups at birth.Furthermore, the ERα mRNA expression was lower in CAF group. These resultssuggest that CAF diet alters the fetal and placental development with adown regulation of ERα mRNA expression in placental tissues.