?mRNA RELATIVE EXPRESSION OF ESTROGEN RECEPTORS α AND β IN PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMC) OF HEALTHY DONORS

LUQUE, ENRIQUE H.; TSCHOPP, MARÍA V.; CARDOZO, MA; RAMOS, JORGE G.; GAYDOU, L

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAIC

Estrogens regulate and play an important role in several physiological and pathological processes. The cellular effects produced by estrogens are mediated by two receptors: ERα and ERβ. The analysis of the ERs expression in PBMC may provide a useful tool to understand the responsiveness of these cells to estrogens and its association with certain pathologies, like chronic lymphocytic leukemia (CLL). In this study we investigated the relative expression of mRNA of both ERs in PBMC from healthy males and females between 25-90 years old. After informed consent, peripheral blood samples were obtained and PBMC isolates using Ficoll?s gradient. RNA was obtained with Trizol reagent, and reverse transcribed. Real-Time PCR was performed using L19 as a housekeeping gene and statistical analyses were carried out using R software. We studied two groups of healthy donors for both genes: men and women younger than 40 and older than 60 years old. With regards to the ERs expression in women we found a significant difference only in the ERβ expression (p=0,0312), being higher levels for those donors older of 60. In males samples, there were significant differences in the two receptors and the higher levels for both were in donors 60 than men of the same age (p=0,0037). Moreover, ERβ showed higher expression in men