mRNA relative expression of estrogen receptors in peripheral blood mononuclear cells (pbmc) of healthy donors.

MA. ALEJANDRA CARDOZO,; JORGE G. RAMOS. ; LUISA GAYDOU; ENRIQUE H. LUQUE; MA. VIRGINIA TSCHOPP

Congreso; Reunión Conjunta de Biociencias.; 2017

Estrogens regulate and play an important role in several physiological andpathological processes. The cellular effects produced by estrogens are mediatedby two receptors: ERα and ERβ. The analysis of the ERs expression in PBMC mayprovide a useful tool to understand the responsiveness of these cells to estrogensand its association with certain pathologies, like chronic lymphocytic leukemia(CLL). In this study we investigated the relative expression of mRNA of both ERs inPBMC from healthy males and females between 25-90 years old. After informedconsent, peripheral blood samples were obtained and PBMC isolates using Ficoll?sgradient. RNA was obtained with Trizol reagent, and reverse transcribed. RealTime PCR was performed using L19 as a housekeeping gene and statisticalanalyses were carried out using R software. We studied two groups of healthydonors for both genes: men and women younger than 40 and older than 60 yearsold. With regards to the ERs expression in women we found a significant differenceonly in the ERβ expression (p=0,0312), being higher levels for those donors olderof 60. In males samples, there were significant differences in the two receptors andthe higher levels for both were in donors 60 than menof the same age (p=0,0037). Moreover, ERβ showed higher expression in men