Uterine proliferative changes in rats exposed to a cafeteria diet after weaning.

MA. PAULA GASTIAZORO, ; MA. FLORENCIA ANDREOLI, ; ENRIQUE H. LUQUE,; GISELA P. LAZZARINO, ; MILENA L. DURANDO,; MARLISE L. GUERRERO SCHIMPF,; JORGE G. RAMOS,; JORGELINA G. VARAYOUD

Mar del Plata, Argentina

Otro; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clinica.; 2016

The development of endometrial cancer is associated Yithobesity. A model of obesity that more closely reflects Yestern diethabits is the cafeteria CAF diet. The CAF diet effects on uterineproliferative response is unMnoYn. The aim of this study Yas toevaluate the CAF diet effects onuterine morphology, proliferationand estrogen-related proteins on adult rats.TYentyonedayold female 9istar rats Yere fed after YeaningYith a standard rodent choY diet control group, C or cafeteriadiet CAF group, Yith highly palatable energy dense foods that areprevalent in Yestern society. The animals Yere YeeMly Yeightedand sacrificed on the first day of diestrousstage, YeeMs afterCAF treatment. $lood Yas collected to determine metabolic parametersand estradiol levels and the uterus Yas removed andincluded in paraffin for histological and immunohistochemicalstudies. Estrogen 4eceptor alpha E4a, 8imentin 8im and -iproliferation marMer Yere determined using immunohistochemistry.The CAF group shoYed obesity after YeeMs of treatmentp.. The CAF diet did not alter serum levels of glucose, cholesterol,triglycerides, insulin and estradiol, but leptin levels Yereincreased C .v. vs CAF .v. ng/ml, p.. The uterusof CAF group shoYed morphological changes Yith an increase ofglandular and stromal areas.+n addition, CAF group shoYed higher 8im expression in thesubepithelial and periglandular stroma. An up regulation of E4aYas detected in all uterine compartments luminal epithelium C.v. vs CAF .v. glandular epithelium C .v. vs CAF.v. subepithelial stroma C .v. vs CAF .v. p..Similar to E4a, -i expression Yas increased in luminal epitheliumC .v. vs CAF .v. and glandular epithelium C.v. vs CAF .v. p..