Perinatal exposure to bisphenol Aimpaired uterine angiogenesis and resulted in intrauterine growth restriction

MEYER N; ZENCLUSSEN AC; SANTAMARÍA, CG; MULLER JE; RODRÍGUEZ HORACIO ADOLFO

Copenhagen

Congreso; 9th Copenhagen Workshop on Endocrine Disrupters; 2017

COW

Xenoestrogenslike bisphenol A (BPA) are proven to have adverse effects on reproductive parameters,like sperm and oocyte quality. However, little is known about the consequences ofexposure to xenoestrogenson endocrine and immunological factors that arepivotal for pregnancy.FemaleC57BL/6 mice were paired with BALB/c males. The day of plug was defined asgestation day (gd) 0. Fromgd 1, females were given50 µg/kg/d BPA daily for 7days (orally). Control mice received 0,001%ethanol.The number of implantation sitesand the abortion rate in each group was determined atgd 5 and14 respectively.Weight of fetuses and placentas wasrecorded and the fetal development wasassessed by high frequency ultrasound. The remodeling of uterine spiralarteries (SA) was studied in HE-stained slides of transversal sections ofimplantations.BPAtreatment did not affect implantationas studied atgd 5.Fetuses of BPA-treatedmothers showed impaired SA remodeling compared to the controls, which resultedin 41 % of fetuses being intrauterine growth restricted at gd 14.We propose that BPA has a profound negative impacton pregnancy, impairing SA remodeling that restricts the maternal blood flowtowards the fetus and results in intrauterine growth restriction. This stronglyencourages the study of the mechanisms involved in BPA effects.