Long term adverse effects on female fertility by neonatal exposure to endosulfan and glyphosate in rats.

MA. MERCEDES MILESI, ; MARLISE L. GUERRERO SCHIMPF, ; ENRIQUE H. LUQUE,; PAOLA I. INGARAMO,; JORGELINA G. VARAYOUD.

Mar del Plata, Argentina

Otro; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clinica.; 2016

2esticide exposure has been associated Yith increased risMof longlasting female reproductive disorders such as alteredcyclicity, decreased conception rates and increased pregnancyloss. HoYever, the impact of the exposure to pesticide mixtureshas received little attention. The aim ofthe present study Yas toevaluate the reproductive effects of neonatal exposureto a mixtureof loY doses of endosulfan and glyphosate commercial formulations.0eYborn female rats received on postnatal days 20& , ,, and , by s.c injection, a commercial formulation of endosulfanat 600 zg/Mg/day E0&, n, a glyphosate based herbicide at mg/Mg/day )$H, n or a mixture of both pesticides at thebefore mentioned doses E0&)$H, n. The control animalsYere injected Yith the vehicle C. 1n 20&, rats Yere matedYith males of proven fertility and the pregnancy rate Yas evaluated.Spermpositive females Yere sacrificed on gestational day to assess the folloYing reproductive parameters number of corpora lutea (nCL), implantation sites (nIS) and resorption sitesn4S. The E0&group shoYed a decrease of the n+S Yhile the)$H exposed animals evidenced a higher n4S Yhen comparedYith the control group. Finally, the binary mixture E0&)$Hrevealed an increase in the n4S, as observed in )$Hgroup.&evelopmental exposure to E0& and )$H commercial formulationsinduced subfertility as a consequence of preimplantationand postimplantation loss, respectively. Coexposure to bothformulations led to postimplantation loss indicating a predominanteffect of )$H formulation. These results draY attention tothe need of more studies on the potential reproductive impactof pesticide mixtures.