Effects of prepubertal exposure to androgens on histofunctional development of the rat uterus.

CARLA E. WAIGANDT ,; GABRIELA A. ALTAMIRANO,; MÓNICA M. MUÑOZ-DE-TORO, ; ENRIQUE H. LUQUE, ; GISELA S. BRACHO, ; LAURA KASS,; VERÓNICA L. BOSQUIAZZO.

Mar del Plata, Argentina

Otro; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clinica.; 2016

The polycystic ovary syndrome 2C1S is a disease thatcan affect Yomen in the reproductive age. +t is characterized foranovulation, hyperandrogenism and polycystic ovaries. 2C1S isassociated Yith an increased risM of endometrial cancer and fertilityproblems. This study Yas performed to evaluate the effects ofhyperandrogenism on the histofunctional development of the ratuterus. To induce 2C1S, 9istar rats Yere injected sc Yith sesameoil control group or &ehydroepiandrosterone &HEA, mg/g body Yeight from postnatal day 20& to 20&. $eforesacrifice, the animals received an ip injection of $romodeoxyuridine$rd7, mg/ g body Yeight. The uterine horns Yere dissectedand included in paraffin. The thicMness of the subepithelialand myometrial stroma, as Yell as, the density of the uterineglands and the nuclei density of the subepithelial and stromalcompartments Yere evaluated in the histological sections. $rd7incorporation and androgen receptor A4 expression Yere alsoquantified in all uterine compartments. The &HEA group shoYedan increment in the subepithelial control .v.um vs &HEA.v.um p. and myometrial control .v.um vs&HEA .v.um p . thicMness, and in the uterine glanddensity control .v.vs &HEA .v. p.. 1n the otherhand, the nuclei density in both compartments Yas decreasedin &HEA animals compared to control rats. The proliferation ofthe subepithelial stroma Yas higher in the &HEA than in controlanimals control .v. vs &HEA .v. p.. Also,&HEA induces the expression of A4 in the cytoplasm of luminaland glandular epithelial cells and in the nuclei of the subepithelialand myometrial stroma. The present study provides evidencethat &HEA treatment induce changes in uterine histomorphologyand steroid receptor expression that could have longtime consequenceson fertility and/or uterine anomalies.