Expression of proteins involved in uterine development and differentiation.

MA. MERCEDES MILESI ,; RAMIRO ALARCÓN, ; NORBERTO M. BELMONTE, ; GISELA H. DIOGUARDI ,; ENRIQUE H. LUQUE; OSCAR E. RIVERA ,; MÓNICA M. MUÑOZ-DE-TORO ,

Mar del Plata, Argentina

Otro; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clinica.; 2016

)lyphosatebased herbicides )$H are one of the most employedagrochemicals around the Yorld. 4ecently, Ye shoYedthat, in the rat, developmental exposure to a )$H alters uterineorganogenetic differen)lyphosatebased herbicides )$H are one of the most employedagrochemicals around the Yorld. 4ecently, Ye shoYedthat, in the rat, developmental exposure to a )$H alters uterineorganogenetic differentiation shoYing hyperplasia in prepubertalsamples and inducing postimplantation embryo loss. 0o reportsare available regarding )$H effects on the reproductive system ofthe eYe lamb. This study investigates the effects of a brief postnatalexposure to a )$H on the morphology and differentiation ofthe prepubertal sheep uterus. EYe lambs Frizone breed Yere scinjected from postnatal day 20& to 20& Yith saline solutionvehicle or mg/-g/day of a )$H. 1n 20& eYe lambs Yerehysterectomized and uterine samples Yere paraffinembedded.+n trichrome stained sections morphological parameters Yereevaluated luminal epithelial height, glandular density, thicMnessof the subepithelial stroma and myometrium. +mmunohistochemistryYas performed to quantify the uterine expression of -icellular proliferation marMer and proteins involved in uterinedevelopment and differentiation estrogen receptor α (ERα), progesteronereceptor (PR), Wnt7a and Ecatenin. )$H treatmentdid not alter uterine morphology, but reduced the proliferationrate in the subepithelial stroma, and in the luminal and glandularepithelium. The decrease in cell proliferation Yas associated Yitha doYnregulation of E4α expression in the same compartments.24 expression Yas increased in the glandular epithelium and in thesubepithelial stroma, and doYnregulated in the luminal epithelium.)$H group also shoYed a decrease in 9nta expression in thesubepithelial stroma and a doYnregulation in Ecatenin expressionin the luminal and glandular epithelium. Exposure to a loY doseof a )$H during early postnatal development decreased uterineproliferation and disrupted the expression of morphoregulatoryproteins in prepubertal sheep. All these alterations might impairfemale fertility at adulthood.tiation shoYing hyperplasia in prepubertalsamples and inducing postimplantation embryo loss. 0o reportsare available regarding )$H effects on the reproductive system ofthe eYe lamb. This study investigates the effects of a brief postnatalexposure to a )$H on the morphology and differentiation ofthe prepubertal sheep uterus. EYe lambs Frizone breed Yere scinjected from postnatal day 20& to 20& Yith saline solutionvehicle or mg/-g/day of a )$H. 1n 20& eYe lambs Yerehysterectomized and uterine samples Yere paraffinembedded.+n trichrome stained sections morphological parameters Yereevaluated luminal epithelial height, glandular density, thicMnessof the subepithelial stroma and myometrium. +mmunohistochemistryYas performed to quantify the uterine expression of -icellular proliferation marMer and proteins involved in uterinedevelopment and differentiation estrogen receptor α (ERα), progesteronereceptor (PR), Wnt7a and Ecatenin. )$H treatmentdid not alter uterine morphology, but reduced the proliferationrate in the subepithelial stroma, and in the luminal and glandularepithelium. The decrease in cell proliferation Yas associated Yitha doYnregulation of E4α expression in the same compartments.24 expression Yas increased in the glandular epithelium and in thesubepithelial stroma, and doYnregulated in the luminal epithelium.)$H group also shoYed a decrease in 9nta expression in thesubepithelial stroma and a doYnregulation in Ecatenin expressionin the luminal and glandular epithelium. Exposure to a loY doseof a )$H during early postnatal development decreased uterineproliferation and disrupted the expression of morphoregulatoryproteins in prepubertal sheep. All these alterations might impairfemale fertility at adulthood.