Long-term effects of neonatal exposure to environmental relevant dose of glyphosate-based herbicide on uterine decidualization

MARLISE L. GUERRERO SCHIMPF,; ENRIQUE H. LUQUE; JORGELINA G. VARAYOUD,; MÓNICA M. MUÑOZ-DE-TORO,; PAOLA I. INGARAMO, ; MA. MERCEDES MILESI,

Otro; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clinica (SAIC).; 2016

+n Argentina, glyphosatebased herbicides )$H are commonlyused to control Yeeds. Although regulatory agencies haveasserted that are relatively safe, reports from our laboratory suggestthat )$H may have endocrine disrupting effects. The aim ofpresent YorM Yas to evaluate the longterm effects of neonatalexposure to a )$H assessing parameters related to uterine decidualizationin rats. Female Wistar pups received saline solutioncontrol, C or an environmental relevant dose of )$H mg/Mgby sc injection on postnatal day 20& , , and . 1n 20&female rats Yere mated Yith fertile nonexposed male. 2regnantrats Yere sacrificed on gestation day )& and )& to evaluatemolecular markers in the implantation sites (IS). Desmin immunostainingYas used as a marMer of decidualization. The neYlyformed blood vessels Yere detected by nestin immunostaining.The 9nta, 9na and Ecatenin expressions Yere evaluated in+S for their Mey roles on decidualization. 1n )&, )$H groupshoYed a high incidence of fetal resorptions. 1n )&, a significantdecrease in the decidualized area &A C .v. mm vs)$H .v. mm, p. linMed to a decreased caliber ofneYly formed vessels Yas found at the +S of the )$H group C.v. zm vs )$H .v. zm, p.. /oreover, 9ntaexpression decreased on antimesometrial zone of +S of )$H groupC .v. vs )$H .v., p. and 9nta expressionYas doYnregulated in luminal epithelium of mesometrial zoneC .v. vs )$H .v., p.. 0o changes Yereobserved in Ecatenin expression betYeen the groups. Longtermeffect of neonatal exposure to )$H Yas evidenced by an increasedincidence of abortion associated Yith altered decidualizationprocess. A decreased &A associated Yith impaired blood supplyin )$H exposed rats may be due to an altered 9nts activationpathYay. These mechanisms may be responsible for the highincidence of abortion in )$H treated rats.