Cafeteria diet alters rat uterine morphology and foeto-placental development

GASTIAZORO MP; LAZZARINO GP; STOKER C; RAMOS JG; GUERRERO SCHIMPF M; ANDREOLI MF; ZANARDI MV; LUQUE EH; ROSSETTI MF; SCHUMACHER R; DURANDO M; VARAYOUD J

Congreso; SLIMP 2017.; 2017

Cafeteria diet alters rat uterine morphology and foeto-placental developmentM.P. Gastiazoro, M. Guerrero Schimpf, M.F. Rossetti, Gisela Lazzarino, M.F. Andreoli, R. Schumacher, C. Stoker, M.V. Zanardi, M. Durando, J.G. Ramos, E.H. Luque, J. VarayoudInstitute of Health and Environment of Litoral (ISAL), Faculty of Biochemistry and Biological Sciences, Universidad Nacional del Litoral ? Consejo Nacional de Investigaciones Científicas y Técnicas, Santa Fe, Argentina.Maternal diet may be associated with foetal and placental growth restriction. Objectives: We evaluated the cafeteria diet effects on: 1) adult rat uterine morphology, 2) reproductive performance, 3) foeto-placental growth on gestational day 21 (GD21), 4) weight of pups at birth, and 4) estrogen receptor α (ERα) mRNA expression in placentas. Methods: Female Wistar rats were fed after weaning with a standard rodent chow diet (control (C)) or cafeteria diet (CAF) with highly palatable energy dense foods. Some animals were sacrificed on diestrus, 20 weeks after treatment. The uterus were included in paraffin for histological and immunohistochemical determination of ERα, vimentin and Ki67 (proliferation marker). Then, some animals were mated to evaluate the reproductive performance and to determine foeto-placental weight and ERα mRNA expression on GD21-placentas by QPCR analysis. Results: CAF group showed increased uterine glandular area expressed as volume density (Vv) (C 4.78 ± 0.76 vs CAF 8.27 ± 1.87, P