Effects of intrauterine exposure to benzophenone-3 in lactating murine mammary gland

ALTAMIRANO GA; KASS L; GOMEZ AL; SCHIERANO-MAROTTI G; MUÑOZ-DE-TORO M

Modalidad Virtual

Congreso; Reunión Anual de Sociedades de Biociencias 2021; 2021

Sociedad Argentina de Investigación Clínica

Benzophenone-3 (BP3), an ultraviolet radiation filter commonly used in sunscreens, has been shown to alter mammary gland (MG) development. Previously, we have demonstrated that exposure to BP3 alters beta-casein (CSN2) and alpha-lactalbumin (LALBA) milk protein expression during functional differentiation of the MG in vitro. Here, our aim was to evaluate whether intrauterine exposure to BP3 alters the lactating mammary gland in the F1 female offspring. Pregnant F0 C57BL/6 mice were dermally exposed to vehicle (sesame oil; Control), 0.15 (0.15BP3) or 50mg BP3/kg/day (50BP3) from gestation day 8.5 to 18. At 8 weeks-old, female offspring (F1) were bred and MG samples were obtained on lactation day 10. The protein expression of the myoepithelial cell biomarker alpha-smooth muscle actin, CSN2 and LALBA was assessed by immunohistochemistry. The perimeter and area of the alveoli and the myoepithelial linear density were measured to establish either the proportion of large/small alveoli per group or the maturation and differentiation of the myoepithelial cells. CSN2, LALBA and whey acidic protein (WAP) mRNA expression was evaluated by qRT-PCR. The alveolar perimeter in 0.15BP3 animals was the lowest among groups (p