PARADOXICAL ANTINEOPLASTIC EFFECT OF SHIGA TOXIN 2 FROM ENTEROHEMORRHAGIC ESCHERICHIA COLI IN TRIPLE-NEGATIVE BREAST CANCER CELLS

LORENA ZARATE; NOELIA MIRET; JORGE GOLDSTEIN; ALIPIO PINTO; ANDREA RANDI

Congreso; LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2020

Shiga toxin 2 (Stx2) is a virulence factor responsible for hemolytic uremic syndrome. Classically, the Stx2´s cytotoxic effect is mediated by its receptor globotriaosylceramide (Gb3). Furthermore, it has been observed that malignant cells represent a great source of Gb3. One of these Gb3-producing malignant tumors is breast cancer, which is the most common cancer type in women worldwide. Triple-negative breast cancer (TNBC) is the most aggressive and difficult to treat from all breast tumors. Our goal is to determine the antineoplastic effect of Stx2 in the TNBC cell line MDA-MB-231. The non-tumorigenic mammary epithelial cell line NMuMG, which lacks Gb3 (negative control), and VERO cells (positive control) were used. Gb3 expression was immunolocalized in MDA-MB-231 and VERO cells, and Stx2 uptake was also observed in both cell lines, with higher levels in VERO cells (p