SOLID SILICA-FUNCTIONALIZED MAGNETIC NANOPARTICLES AS DRUG

CAMPELO ADRIÁN; AGOTEGARAY MARIELA; LASALLE VERÓNICA; MASSHEIMER VIRGINIA

Mar del Plata

Congreso; LXI Reunión Científica anual de la Sociedad Argentina de investigación Clínica.; 2016

Sociedad Argentina de Investigación Clínica

Introduction: Solid silica (Si) coated magnetic nanoparticles (MNPs) are potential devices for drug targeting. Stability and bio-compatibility of silica turn these systems feasible forbiomedical ap-plications althoughdrug loading is a challenge due to silica inertia. Objective: Synthesis, characterization and Diclofenac (Dic) incor-poration onto citric acid(CA)-functionalized magnetite(MG) coated with Siand 3-aminopropyltriethoxysilane(APTES). Evaluation of cy-totoxicity on rat aortic endothelial cells (EC). Design: MG/CA NPs were obtained by co-precipitation. Si and APTES functionalization was performed bya modified Stöber process, prolonged for 12h at room temperature, using ratios MG/CA:TEOS:APTES=(1:0.5:2; 1:1:2; 1:2:2), named 1, 2, and 3. They were characterized by FTIR, DLS, z potential and HR-TEM microscopy. Dic was bonded by N,N´-dicyclohexylcarbodiimideand studied by UV-Vis spectroscopy. Primary cultures of EC were exposed for 48h to final concentrations of 1, 10 and 100µg/mL of MNPs and Dic-loaded MNPs. Cell viability was studied by MTT assay and by the capacity to produce NO (DAN assay). Results: 1 and 2 presented Dh of 400.0±10.0nm and 520±10.0nm; z of 27.9±5.78mV and21.9±6.02mV in aqueous dispersions. 3 was polydisperse with z 32.6±5.81mV. HR-TEM micrographs showed matrix dispersed structures for the MNPs. Formulation 2 incorporated approx. 40% of Dic. Cell viability was not affected at 10µg/mL or below for all the samples (p