Approach to deliver N-acetylcysteine with silica nanoparticles into cells

MONTALDO LAURA; FRONTERA EVELYN MICAELA; DE MARZI MAURICIO; FRONTERA EVELYN MICAELA; DE MARZI MAURICIO; MITAROTONDA ROMINA; GUERRA LILIANA; MITAROTONDA ROMINA; GUERRA LILIANA; MONTALDO LAURA

FREE RADICAL BIOLOGY AND MEDICINE

ELSEVIER SCIENCE INC

Año: 2019 vol. 139 p. 24 - 25

0891-5849

In our laboratory we evaluated N-Acetylcysteine (NAC) effects by its addition to cell culture media. We demonstrated that NAC inhibits adipogenic differentiation of 3T3-L1 preadipocytes (Soto et al, 2017). Here,we performed treatments with 0.01 to 5 mM NAC, on 3T3-L1 adipocytes(AC). NAC cellular uptake, in 5mM NAC treated 3T3-L1 adipocytes (ACN-5), was only 17.4% of total NAC added. Highest NAC dose decreased totalcholesterol content (Chol) (1.61 0.50 g Chol / g protein [ACN-5] vs5.73 0.02 g Chol/ g protein [AC], p < 0.05). We evaluated cytotoxicityby colorimetric assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide; none dose of NAC was cytotoxic (OD: 0.85 0.02[ACN-5] vs 0.81 0.08 [AC], no significant difference). We developedspherical porous silica nanoparticles (20 4.5 nm), in which weencapsulated 5mM NAC (NPsSiO2-NAC), and evaluated their cytotoxicity.Samples of 0.05mg/mL showed 42.3 % cytotoxicity (OD:0.410.04 [NPsSiO2-NAC] vs 0.710.04 [nontoxic control], p < 0.01);with a cover of bovine sera albumin (8nm) we obtained nontoxic NACnanoparticles(OD: 0.910.09 [NPsSiO2-NAC] vs 0.900.08 [nontoxiccontrol], no significant difference). NAC release was completed within28 hours. This tool for NAC delivery could increase NAC cellularuptake and its effect on lipid content.