INVESTIGADORES
ROMEO Horacio Eduardo
artículos
Título:
IL-17 and IFN- expression in lymphocytes from patients with active tuberculosis correlates with the severity of the disease
Autor/es:
JAVIER O. JURADO, VIRGINIA PASQUINELLI, IVANA B. ALVAREZ, DELFINA PEÑA, ANA I. ROVETTA, NANCY L. TATEOSIAN, HORACIO E. ROMEO, ROSA M. MUSELLA, DOMINGO PALMERO, H. EDUARDO CHULUYÁN, VERÓNICA E. GARCÍA
Revista:
JOURNAL OF LEUKOCYTE BIOLOGY
Editorial:
FEDERATION AMER SOC EXP BIOL
Referencias:
Lugar: Bethesda; Año: 2012 vol. 91 p. 991 - 1002
ISSN:
0741-5400
Resumen:
Th1 lymphocytes are crucial in the immune responseagainst Mycobacterium tuberculosis. Nevertheless,IFN- alone is not sufficient in the complete eradicationof the bacteria, suggesting that other cytokines mightbe required for pathogen removal. Th17 cells have beenassociated with M. tuberculosis infection, but the roleof IL-17-producing cells in human TB remains to be understood.Therefore, we investigated the induction andregulation of IFN- and IL-17 during the active disease.TB patients were classified as High and Low Responderindividuals according to their T cell responsesagainst the antigen, and cytokine expression upon M.tuberculosis stimulation was investigated in peripheralblood and pleural fluid. Afterwards, the potential correlationamong the proportions of cytokine-producingcells and clinical parameters was analyzed. In TB patients,M. tuberculosis induced IFN- and IL-17, but incomparison with BCG-vaccinated healthy donors, IFN-results were reduced significantly, and IL-17 was markedlyaugmented. Moreover, the main source of IL-17was represented by CD4IFN-IL-17 lymphocytes, aTh1/Th17 subset regulated by IFN-. Interestingly, theratio of antigen-expanded CD4IFN-IL-17 lymphocytes,in peripheral blood and pleural fluid from TB patients,was correlated directly with clinical parametersassociated with disease severity. Indeed, the highestproportion of CD4IFN-IL-17 cells was detected inLow Responder TB patients, individuals displaying severepulmonary lesions, and longest length of diseaseevolution. Taken together, the present findings suggestthat analysis of the expansion of CD4IFN-IL-17 Tlymphocytes in peripheral blood of TB patients might beused as an indicator of the clinical outcome in activeTB.
