Variation of sperm motility and mitocondrial potential parameters due to the antioxidant effect of trolox on boar semen refrigeration

CÓRDOBA M; CAMPORINO AGUSTINA

reunion virtual

Congreso; IV Reunión Conjunta de Sociedades de Biología de la República Argentina ?Nuevas evidencias y cambios de paradigmas en ciencias biológicas; 2020

Sociedades de Biología de la República Argentina

VARIATION OF SPERM MOTILITY AND MITOCONDRIAL POTENTIAL PARAMETERS DUE TO THE ANTIOXIDANT EFFECT OF TROLOX ON BOAR SEMEN REFRIGERATIONCamporino A, Córdoba MInstituto de Investigación y Tecnología en Reproducción Animal (INITRA, UBA),Unidad ejecutora de Investigaciones en Producción Animal (INPA, UBA-CONICET),Cátedra de Química Biológica, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Argentina. mcordoba@fvet.uba.arGamete cryopreservation is a fundamental biotechnological strategy in the productive field. However in swine production, it is not regularly used due to the high concentration of unsaturated fatty acids makes boar sperm more susceptible to peroxidation produced by reactive oxygen species generated during their storage at low temperatures. Our aim was to study the effect of the addition of the synthetic compound derived from alpha-tocopherol called 'Trolox' to Modena modified diluent in porcine semen by means of functional sperm tests: evaluation of sperm motility, pre-capacitation status, sperm viability, membrane integrity, and mitochondrial membrane potential. We worked with a pool of semen samples that were separated into two equal aliquots, only one of them treated with Trolox, and both stored at 17°C. Both samples were evaluated on days 0 (fresh semen), 1, 2 and 5 of refrigeration. Subsequently, motility changes were studied using the Integrated Sperm Analysis System (software version 1.2, PROISER). Pre-capacitation was evaluated by the chlorotetracycline epifluorescent technique and viability by trypan blue staining. The functional integrity of the plasma membrane was evaluated through the Hypoosmotic Test, while the mitochondrial membrane potential was evaluated through the JC-1 fluorochrome. Data were analyzed by ANOVA/ Tukey test (P